PROSPORE GLASS AMP BIO-IND BX/10

HealthLink offers a complete line of biological indicators to meet all your sterilization assurance needs. Follow the link below to view or download literature on ProSpore or ProSure® products.

INSTRUCTIONS FOR USE :

1. Exposure:
Place one or more ProSpore Biological Indicators in the most difficult location to sterilize (usually near the drain or suspended in a volume of liquid). Run Cycle.

Caution: After sterilization, handle ampules with care. Contents of the ampule are hot and under pressure. Failure to allow sufficient cooling time (10-15 minutes) may result in bursting of the ampule.

2. Incubation:
Place the ampule in a vertical position in an incubator maintaining 55o-60oC. Mark a control ampule as such and incubate along with processed ampules to ensure spore viability. HealthLink recommends an incubation time of 48 hours.


3. Monitoring:
Examine the ProSpore ampules daily during incubation. Record observations. All Positive vials should be recorded and then disposed of immediately.

4. Interpretation:

* Control: The control vial should exhibit a color change and/or turbidity. If the control ampule does not show signs of growth, consider the test invalid.
* Test: A failed sterilization cycle is indicated by turbidity of a change in color to or toward yellow. A test ampule that retains its purple color indicates an adequate sterilization cycle.
* Resistance Characteristics: In saturated steam at 1210C:
* Survival Time (in minutes) = not less than (labeled D-value) x [(log10 labeled spore count) - 2]*
* Kill time (in minutes) = not more than (labeled D-value) x [(log10 labeled spore count) + 4]*